Table 3.

MIC^† of fluorescein analogs on growth of microbes in plate assay

Bacteria	E. coli MC4100	E. coli NR698	B. subtilis 168
Chemical*
Rose Bengal (RB)	>1 mM	3.1 µM	3.1 µM
Erythrosin B (EB)	>10 mM	250–500 µM	250–500 µM
Diiodofluorescein (DI)	>3 mM	200–500 µM	1 mM
Ecosin Y (EY)	NA	1–2.5 mM	2.5 mM
Dinitrofluorescein (DN)	NA	10–20 mM	10 mM

^†Minimal inhibition concentrations (MIC) were determined by the concentration of inhibitors showing a clear zone after overnight incubation as compared to the surrounding area.

^*Fluorescein analogs were applied to the bacterial cells as described in Material and Methods.