Figure 7. Chronic cuprizone treatment does not result in collagen deposition.

Masson trichrome histological stain to detect collagen fibrils (blue) along with cytoplasm (pink) and muscle fibres (red) in a positive control section through a segment of intestine (A). In coronal sections through the corpus callosum (cc) from normal adult mice (B, 8 weeks) myelin also appears pink with the trichrome stain and blue nuclei are visible but collagen fibrils are not present. After 12 weeks of cuprizone (C) loss of the pink myelin staining is evident throughout the corpus callosum and nuclei are less organized but parenchymal or perivascular accumulations of collagen fibrils are not observed. Scale bars in (A–C) = 200 μm.