Relative expression levels of GS biosynthetic and regulatory genes in trichomes of wild-type plants (Col-0). (A) Relative expression level of CYP79B3, MAM1, CYP83B1, CYP83A1, TGG1, GL2, and LHCBI in trichomes of wild-type plants; (B) Relative expression of indolic glucosinolate (IGS) regulators: MYB51/HIG1, MYB34 and MYB122;
(C) Relative expression of aliphatic glucosinolate (AGS) regulators: MYB28, MYB29 and MYB76. Expression analysis of genes required for GS biosynthesis was performed on detached trichomes, which were collected as described in the Materials and Methods. GLABRA2 gene (GL2), which is mainly found in trichomes and LHCBI, which is expected to be found in photosynthetically active leaf tissue have been used as positive and negative controls for corresponding tissue types. Three biological and two technical replicates were used for the analysis. Error bars show SE of the mean. Relative gene expression levels are shown in trichomes vs. leaves (leaves = 1). Significantly different values in trichomes vs. leaves are marked with asterisk (Student's t-test, P < 0.05).