Table 1.
Comparison of ECL Sensor and LC–MS Nanoreactor Data to in Vivo Toxicity
| substrate (film) | initial slopea | turnover rateb | LC-UV turnoverc | TD50d,e |
|---|---|---|---|---|
| NPYR (RLM) | 0.144 (±0.015) | 781(±176) | 12.9(±2.6) | 1.52 |
| NPYR (h2E1) | 0.216 (±0.021) | 779(±208) | 11.6(±2.4) | |
| NNK (RLM) | 0.116 (±0.004) | 629(±128) | 14.0(±2.8) | 1.80 |
| NNK (h2E1) | 0.230 (±0.029)) | 832(±233) | 10.9(±2.2) | |
| NPIP (RLM) | 0.110 (±0.011) | 594(±132) | 13.4(±2.7) | 1.50 |
| styrene (RLM) | 0.036 (±0.011) | 196(±126) | ndf | 23.3g |
| styrene (h2E1) | 0.051 (±0.017) | 184(±77) | nd |
a
In units of min−1.
b
Relative values from ECL arrays, in min−1 (mg of protein)−1; obtained by dividing initial slope by amount of protein based on QCM (Si, Table S1).
c
Relative values from LC-UV absorbance, in abs. units (normalized protein × s)−1.
d
TD50, chronic dose (mg/kg of body weight per day) inducing mixed liver tumors in half of test rat population at end of standard life span.23
e
Ad libitum in drinking water.
f
Not detected within 30-min reaction time.
g
Gavage administration.