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. Author manuscript; available in PMC: 2013 Sep 20.
Published in final edited form as: Neuron. 2012 Sep 20;75(6):1035–1050. doi: 10.1016/j.neuron.2012.08.031

Figure 2. Mek1/2 deletion disrupts glial progenitor specification.

Figure 2

(A–A′) Expression of Tenascin C, a glycoprotein secreted by astrocyte precursor cells, is strongly reduced in the E18.5 Mek1,2\Nes cortex. (B–B′) BLBP immunostaining revealed a dramatic reduction in astrocyte precursor number in mutant cortex. Scale bar=100 μm. (C–C′) High magnification images of BLBP+ cells from delineated areas in B and B′. BLBP+ cell somas, which represent astrocytes precursors, were nearly absent in the mutant cortex at P0. Scale bars=50μm. (n=3). (D–E′) The numbers of Olig2+ and PDGFRα+ oligodendrocyte progenitor cells are profoundly reduced in the E19.5 mutant dorsal cortex. (n=3). Scale bar=100μm. (F) Western blotting confirms reduced Aldh1l1 and Acsbg1 expression in Mek1,2\Nes dorsal cortex compared to WT. 1 and 2 indicate duplicate protein samples. (mean ± s.e.m; ** = p-value < 0.01, paired t-test). (G) Microarray analysis shows dramatic reductions of mRNAs expressed specifically in glial progenitors in Mek1,2\Nes dorsal cortex, while expression of the neuronal genes shown was not altered. See also Figure S2.