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. Author manuscript; available in PMC: 2012 Oct 30.
Published in final edited form as: Gene Ther. 2010 Apr 29;17(9):1098–1104. doi: 10.1038/gt.2010.57

Figure 1. Stretch-enhanced gene transfer is amplitude dependent.

Figure 1

Cells were grown on Pronectin treated BioFlex culture plates (Flexcell International Corporation, Hillsborough, NC) and electroporated with 10 μg of pCMV-Lux-DTS. The cells were then stretched for 30 minutes at either 0%, 4%, 10% or 20% change in basement membrane area (0.5 Hz, 50% duty cycle) followed by static growth for 23.5 hours. After 24 hours, luciferase expression was determined (n = 6 for each condition). *, p < 0.05, **, p < 0.01 between stretched and unstretched pairs.