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. Author manuscript; available in PMC: 2012 Oct 30.
Published in final edited form as: Gene Ther. 2010 Apr 29;17(9):1098–1104. doi: 10.1038/gt.2010.57

Figure 2. Mechanical ventilation increases gene transfer and transgene expression.

Figure 2

Fifty μg of pCMV-Lux-DTS were instilled into the lungs of anesthetized Balb/c mice via an endotracheal tube and electroporated within one minute of DNA delivery. Immediately following electroporation, animals were subjected to no mechanical ventilation (“NMV”) or ventilation at 150 breaths per minute at the indicated volumes for (A) 5 min or (B) 20 min, and then allowed to recover. Gene expression was measured in the lungs 2 days later (n = 5 for each condition). *, p< 0.5 vs. not ventilated; **, p<0.01 vs. not ventilated; ***, p<0.001 vs. not ventilated.