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. Author manuscript; available in PMC: 2012 Oct 30.
Published in final edited form as: Gene Ther. 2010 Apr 29;17(9):1098–1104. doi: 10.1038/gt.2010.57

Figure 3. Enhanced gene transfer requires ventilation following electroporation.

Figure 3

Fifty μg of pCMV-Lux-DTS were instilled into the lungs of anesthetized Balb/c mice via an endotracheal tube (“DNA”), electroporated (“E”), and/or ventilated at 150 breaths per minute at 16 ml/kg for 5 min (“V”) in the order indicated (n = 5 per condition). If not indicated, the time between treatments was less than 60 seconds; where indicated, 10′ represents a 10 minute lag period between treatments. Following treatment, animals were allowed to recover and two days later gene expression was measured in the lungs of the animals. ***, p<0.001 vs. DNA followed immediately by electroporation in the absence of ventilation.