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. Author manuscript; available in PMC: 2012 Oct 30.
Published in final edited form as: Gene Ther. 2010 Apr 29;17(9):1098–1104. doi: 10.1038/gt.2010.57

Figure 4. Mechanical ventilation increases the distribution of gene expression in the electroporated lung.

Figure 4

Fifty μg of pCMV-lacZ-DTS were instilled into the lungs of anesthetized Balb/c mice via an endotracheal tube, electroporated within 60 seconds of DNA delivery, and then either not ventilated (NMV) or ventilated for 5 min at 150 breaths per minute at 16 ml/kg. Gene expression (blue) was evaluated by IHC using a rabbit anti-ß-galactosidase antibody at 2 days post-transfer. (a) Naïve (no DNA transferred), (b) NMV, (c) 16 ml/kg.