Comparison of normalized GFP intensity between native HCs, and transfected GER cells that expressed Pou4f3/GFP alone or Pou4f3/GFP and myosin VIIa. GFP intensities were measured in the samples transfected with 0.5 μg/μl hATOH1 alone (A), hATOH1 plus hTCF3 (AT), and hATOH1 plus hTCF3 plus hGATA3 (ATG). GER cells that expressed both Pou4f3/GFP and myosin VIIa exhibited significantly greater GFP intensity than cells that expressed only Pou4f3/GFP. While the intensity of Pou4f3/GFP+/myosin VIIa+ GER cells was similar to that of native HCs, there were no differences of GFP intensity of GER cells among the three transfection groups.
* indicates p<0.001 with the Mann-Whitney’s U test using Bonferroni correction for multiple comparison. Abbreviations are the same as those of Fig. 8.