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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Dev Biol. 2012 Sep 15;372(1):68–80. doi: 10.1016/j.ydbio.2012.09.002

Fig. 8. Quantitative analysis of Pou4f3/GFP induction by TF co-transfection.

Fig. 8

The numbers of Pou4f3/GFP+ cells observed in the GER following transfection with plasmids encoding TFs, alone or in combination. The numbers of Pou4f3/GFP+ GER cells observed along the entire length of the sensory epithelium (a), in the apical turn (b), in the middle turn (c), or in the basal turn (d) are shown separately. Transfection of hATOH1 alone and hTCF3 alone induced Pou4f3/GFP+ GER cells, but hGATA3 alone did not. Co-transfection of hATOH1 with either hTCF3 or hGATA3 induced more Pou4f3/GFP+ GER cells, and the increase was greater than that induced by hATOH1 alone or hTCF3 alone in the entire cochlea (a) and apical turn (b), although the effect of adding hGATA3 to hATOH1 was not significant statistically in the basal turn, while the triple combination of hATOH1, hTCF3 and hGATA3 was required to increase Pou4f3/GFP+ GER cells significantly over that observed with hATOH1 alone in the middle turn. These indicate that transfection of hATOH1 in combination with either hTCF3 or hGATA3 enhances the hATOH1 effect on 8.5 kb of 5′ Pou4f3 regulatory DNA synergistically.

0.5 A, 0.5 μg/μl of hATOH1 expression plasmid; 1.0 A, 1.0 μg/μl of hATOH1 expression plasmid; T, 0.5 μg/μl of hTCF3 expression plasmid; G, 0.5 μg/μl of hGATA3 expression plasmid; S, 1.0 μg/μl of hSP1 expression plasmid; P, 0.5 μg/μl of empty vector.

# or ## indicates a significant difference at p<0.05 or p<0.01 from 0.5 μg/μl of hATOH1 alone by one-way ANOVA with Dunnett’s multiple comparison test. *or ** indicates a significant difference at p<0.05 or p<0.01 from 1.0 μg/μl of hATOH1 alone with the same test.