Table 1.
Effect of different concentrations of ethylene inhibitors on on shoot regeneration and growth from leaf cultures of Sinningia speciosa after 6 weeks in culture on regeneration medium (MS medium with 2.0 mg/L BA and 0.1 mg/L NAA).
|
Ethylene inhibitors* (mg L−1) |
Regeneration frequency** (%) |
Number of shoots per explant** | Shoot lengtha
(cm) |
|
|---|---|---|---|---|
| Control | 0 | 72 | 12.3 ± 0.8 | 1.21 ± 0.1 |
|
| ||||
| AVG | 1 | 85 | 14.3 ± 1.2 | 1.23 ± 0.2 |
| 5 | 75 | 13.5 ± 0.9 | 1.6 ± 0.2 | |
| 10 | 64 | 10.6 ± 0.7 | 1.8 ± 0.2 | |
| 20 | 58 | 8.2 ± 0.4 | 1.1 ± 0.1 | |
|
| ||||
| CoCl2 | 1 | 79 | 13.8 ± 0.7 | 1.36 ± 0.1 |
| 5 | 72 | 11.8 ± 0.9 | 1.18 ± 0.1 | |
| 10 | 62 | 9.2 ± 0.6 | 0.85 ± 0.1 | |
| 20 | 45 | 5.9 ± 0.4 | 0.57 ± 0.1 | |
|
| ||||
| STS | 1 | 77 | 13.1 ± 0.9 | 1.18 ± 0.1 |
| 5 | 91 | 17.2 ± 1.6 | 1.27 ± 0.2 | |
| 10 | 89 | 16.8 ± 1.2 | 1.25 ± 0.2 | |
| 20 | 63 | 11.4 ± 0.8 | 0.84 ± 0.1 | |
∗Basal medium consisted of MS salts and vitamins, 30 g L−1 sucrose, and 2 mg/L BAP, solidified with 7 g/L Phytagar.
∗∗From 100 leaf explants tested.
aValues represent the mean ± standard deviation of 50 shoots.