Skip to main content
. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Methods Mol Biol. 2013;931:29–59. doi: 10.1007/978-1-62703-056-4_2

Fig. 7.

Fig. 7

Images of (a) DAPI (Zeiss 49 DAPI cube; 250 ms), (b) phalloidin AlexaFluor® 488 (Zeiss 13 FITC cube; 400 ms), and (c) MitoTracker® Red CMXRos (Chroma Technology ET-Texas Red cube, 600 ms) taken on the same microscope with the same camera settings as in Fig. 2. The mercury lamp was attenuated to 5% power with neutral density filters. Z-axis images were taken every 0.1 μm for a total of 82 image frames. (a–c) Images are maximum projections of nine images centered about the focal plane for the raw widefield images (first column), and images deconvolved with AutoQuant X (third column). The second and third columns are displayed with the same brightness, contrast, and gamma factor settings to show the increase in S/N with deconvolution. The gamma factor was used to bring up dim features so they are visible in the images. The first column is displayed with enhanced brightness and contrast so that image resolution can be visualized. Scale bar is 10 μm.