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. Author manuscript; available in PMC: 2013 Jun 11.
Published in final edited form as: Angew Chem Int Ed Engl. 2012 Apr 27;51(24):5876–5879. doi: 10.1002/anie.201200526

Figure 2.

Figure 2

Mg2+-dependent folding and dynamics of minimal hTR pseudoknot domain. a) FRET distribution for minPKWT in the absence (left panel) or presence (right panel) of 1 mM Mg2+. b) Representative single molecule FRET trace of minPKWT in the absence of Mg2+. The abrupt loss of signal intensity at the end of the trace is due to photobleaching of the dye. Hidden Markov Modeling (HMM)[18] of the FRET trajectories yielded idealized state traces (magenta) which were used to calculate transition rates between observed FRET states (Supplementary Figure 3). c) FRET distribution for minPK DKC in the absence (left panel) or presence (right panel) of 1mM Mg2+. d) Representative single molecule trace of minPKDKC in the absence of Mg2+.