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. Author manuscript; available in PMC: 2013 Jun 11.
Published in final edited form as: Angew Chem Int Ed Engl. 2012 Apr 27;51(24):5876–5879. doi: 10.1002/anie.201200526

Figure 4.

Figure 4

Mg2+-dependent folding of the nearly full-length hTR PK domain. a) Schematic illustration of the fullPKWT smFRET construct sequence with FRET dye attachments and DNA handle attached as described for the minPK WT. b) FRET distributions for fullPKWT as a function of [Mg2+].c) FRET distributions for the fullPKDKC as a function of [Mg2+]. d) Quantification of pseudoknot formation at varying Mg2+ concentrations. The fraction of fullPKWT (blue) or fullPKDKC (red) exhibiting PK folding. Error bars represent the standard deviation of experiments done in triplicate.