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. Author manuscript; available in PMC: 2012 Oct 30.
Published in final edited form as: Leukemia. 2011 Jun 10;25(10):1610–1619. doi: 10.1038/leu.2011.120

Figure 4. Induction of HLA-A2-restricted and XBP1-specific cell proliferation of XBP1-CTLs in response to multiple myeloma cells.

Figure 4

Proliferation of CFSE-labeled XBP1-CTLs was measured in response to antigen stimulation by flow cytometry on day 4 of culture. The cell proliferation of heteroclitic XBP1184–192 (YISPWILAV) and heteroclitic XBP1 SP367–375 (YLFPQLISV) CTLs were both XBP1 antigen-specific and HLA-A2-restricted in response to the McCAR (HLA-A2+/XBP1+) myeloma cell line. The CTLs did not proliferate in response to either the XBP1 antigen or MHC mismatched cell lines ML-2 (HLA-A2+/XBP1−) or MM1S (HLA-A2−/XBP1+), respectively. Background proliferation was determined using CFSE labeled heteroclitic XBP1-CTLs cultured in media alone. The results are representative of three independent experiments.