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Analysis of polypeptides by SDS-PAGE of the purification steps of NADP+-IDH. Samples with 20, 10, 5, and 0.5 μg of proteins corresponding to the crude extract, ammonium sulfate precipitate, DEAE-cellulose, and Matrex Gel Red A, respectively, were separated by SDS-PAGE and stained with silver. The positions of molecular mass standards are indicated on the left. The migration of the NADP+-IDH subunit is marked on the right.
