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Purification of NADP+-IDH from maritime pine cotyledons
| Purification Step | Total Activity | Total Protein | Specific Activity | Yield | Purification |
|---|---|---|---|---|---|
| nkat | mg | nkat mg−1 protein | % | -fold | |
| Crude extract | 240 | 133 | 1.8 | 100 | 1 |
| 45% to 85% (NH4)2SO4 | 200 | 12 | 16.6 | 83 | 9.2 |
| DEAE-cellulose | 160 | 21 | 76.1 | 66 | 42.3 |
| Matrex Gel Red A | 90.3 | 0.2 | 451.5 | 37.6 | 250.8 |
Data are representative of five different purifications from 50 g of tissue from seedlings with cotyledons 1 to 2 cm in length.
