Figure 5.

Large oncosome-like vesicles in metastatic prostate cancer. A: Representative paraffin section of human core biopsy specimens of patients with prostate cancer (Gleason score, 4 + 3) are immunostained with CK18 antibody. Arrowheads, structures resembling large oncosomes. B: Quantitative analysis of the distribution of ARF6-positive large oncosome-like vesicles among the diagnostic categories, showing vesicles are significantly more abundant in those with a Gleason score of >7 than a Gleason score of ≤7 (P = 0.020) and in metastases (Mets) than in organ-confined tumors [prostate cancer (PCa)] (P = 0.001). C: Representative sections of an additional prostate cancer TMA stained with ARF6, showing the absence (left panel) and presence (right panel) of large oncosome-like vesicles (arrowheads). D: The presence of structures resembling large oncosomes significantly discriminates between normal and tumor samples (P < 0.0001) and between organ-confined disease and metastasis (P = 0.0008).