Figure 5.

SCD mice have decreased circulating B cells and increased circulating CD8⁺ T cells and Tregs in peripheral blood. A–C: Lymphocyte flow cytometric analysis on peripheral blood of C57BL/6 (n = 6), hemizygous (n = 6), and SCD (n = 5) mice is performed. Single-cell suspensions are isolated and stained with anti-CD3, anti-CD4, anti-CD25, anti-FoxP3, anti-CD8, anti-CD19, and anti-B220 antibodies. T cells are CD3⁺ lymphocytes. B cells are B220⁺CD19⁺ lymphocytes. CD4⁺ T cells are CD3⁺CD4⁺ lymphocytes. CD8⁺ T cells are CD3⁺CD8⁺ lymphocytes. Tregs are CD4⁺CD25⁺FoxP3⁺ lymphocytes. Bars represent mean ± SEM percentages. Significant differences are determined by one-way analysis of variance with multiple pairwise comparisons between groups using the Newman-Keuls post hoc test. *P < 0.05, **P < 0.01, and ***P < 0.001.