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. 2012 Oct 25;7(10):e48067. doi: 10.1371/journal.pone.0048067

Table 1. PBMC phenotype is affected after cryopreservation.

PBMC	Fresh (%)	Day 1 (%)	Day 28 (%)
*CD31⁺*	67.38±2.17	70.42±4.12	76.88±2.10^a,b
CD31⁻CD34⁺	10.12±0.47	7.69±0.62^a	8.11±0.80^a,b
CD31⁻CD34⁺VEGFR2⁺	0.61±0.14	1.55±0.17	2.16±0.15
CD31⁻VEGFR2⁺	1.64±0.39	3.88±0.42^a	4.91±0.34^a
CD31⁺CD34⁻VEGFR2⁺	3.36±0.57	8.04±0.73^a	5.14±2.28^a
CD31⁺CD34⁻L-Selectin⁺VEGFR2⁺	3.50±0.24	5.12±1.10	6.31±0.70^a
CD31⁺CD34⁺	29.47±0.48	40.13±6.13	38.8±2.42
CD31⁺CD34⁺VEGFR2⁺	3.71±0.30	15.42±5.82	13.30±1.64
CD31⁺L-Selectin⁺	60.89±3.11	60.76±1.04	62.54±1.73
CD31⁺VEGFR2⁺	7.30±0.42	22.02±4.67	22.02±1.0
*CD34⁺*	35.89±1.36	48.14±2.72^a	43.93±2.59^a
CD34⁺L-Selectin⁻	4.72±0.82	5.42±0.89	4.72±1.02
CD34⁺L-Selectin^-VEGFR2⁺	0.61±0.22	3.66±0.61^a	2.58±0.76^a
CD34⁺L-Selectin⁺	27.44±1.58	24.91±0.61	23.39±1.92^a
CD34⁺L-Selectin⁺VEGFR2⁺	2.24±0.58	6.94±0.57^a	4.63±2.15
CD34⁺CD133⁺	0.14±0.053	0.53±.077^a	0.44±0.17
CD34⁺CD133⁺VEGFR2⁺	0.015±0.0082	0.15±0.043^a	0.12±0.049
CD34⁺VEGFR2⁺	2.33±0.56	11.66±2.99^a	7.63±2.81
*L-Selectin⁺*	84.37±1.52	74.79±2.43^a	70.76±2.82^a
L-Selectin⁻VEGFR2⁺	0.75±0.33	4.06±0.25^a	3.57±0.61^a
L-Selectin⁺VEGFR2⁺	3.66±0.56	6.40±0.79	5.08±1.61
*CD133⁺*	0.27±0.067	2.65±0.97^a	1.97±0.23^a
CD133⁺VEGFR2⁺	0.0027±0.0027	0.072±0.013^a	0.084±0.031^a
*VEGFR2⁺*	12.47±2.85	27.57±6.19^a	27.07±5.44^a

^a

p<0.05 vs. ‘fresh’ time point.

^b

p<0.05 vs. ‘day 1’ time point.

The values represent the percentage of cells within the total cell population (sample of 200,000 cells) that express the given phenotype.