Table 2. CAC phenotype is affected after cryopreservation.
CAC | Fresh (%) | Day 1 (%) | Day 28 (%) |
CD31+ | 72.81±2.94 | 61.97±1.09a | 58.10±5.12 |
CD31−CD34+ | 8.38±1.25 | 12.51±0.58a | 12.98±0.78a |
CD31−CD34+VEGFR2+ | 0.79±0.23 | 2.70±0.32a | 1.76±0.53 |
CD31−VEGFR2+ | 1.80±0.50 | 7.35±0.96a | 4.62±1.20 |
CD31+CD34−VEGFR2+ | 3.83±0.66 | 8.83±0.32a | 6.80±0.83a |
CD31+CD34−L-Selectin+VEGFR2+ | 3.05±0.51 | 6.88±0.26a | 4.65±0.72 |
CD31+CD34+ | 31.45±1.68 | 29.20±1.20 | 19.39±3.24a |
CD31+CD34+VEGFR2+ | 3.81±0.63 | 8.42±0.89a | 5.27±0.82b |
CD31+L-Selectin+ | 64.26±2.69 | 58.45±1.36 | 45.79±6.69a |
CD31+VEGFR2+ | 6.75±1.08 | 16.04±1.14a | 11.19±1.63 |
CD34+ | 42.53±1.89 | 42.73±2.17 | 38.51±3.26 |
CD34+L-Selectin− | 4.53±0.82 | 2.69±0.37 | 2.18±0.61 |
CD34+L-Selectin−VEGFR2+ | 0.90±0.19 | 1.95±0.29a | 0.78±0.24b |
CD34+L-Selectin+ | 31.29±1.61 | 28.03±1.06 | 23.78±3.27 |
CD34+L-Selectin+VEGFR2+ | 3.44±0.54 | 9.32±1.16a | 6.31±1.29 |
CD34+CD133+ | 0.24±0.11 | 0.71±0.25 | 4.35±2.43 |
CD34+CD133+VEGFR2+ | 0.070±0.042 | 0.23±0.064 | 0.44±0.22 |
CD34+VEGFR2+ | 3.57±0.63 | 10.07±1.28 | 5.88±0.99 |
L-Selectin+ | 82.99±2.65 | 87.26±1.47 | 92.04±1.98a |
L-Selectin−VEGFR2+ | 1.00±0.31 | 2.88±0.36a | 2.83±0.72a |
L-Selectin+VEGFR2+ | 3.97±0.67 | 10.34±0.67a | 6.32±1.30b |
CD133+ | 2.54±1.15 | 4.93±1.02 | 3.60±1.89 |
CD133+VEGFR2+ | 0.050±0.030 | 0.12±0.022 | 2.39±1.40 |
VEGFR2+ | 14.67±3.15 | 26.26±2.19 | 28.44±6.47 |
p<0.05 vs. ‘fresh’ time point.
p<0.05 vs. ‘day 1’ time point.
The values represent the percentage of cells within the total cell population (sample of 200,000 cells) that express the given phenotype.