Evolutionary relationships inferred from SOD
sequences. Accession numbers for the sequences utilized are listed in
Table II. A, Dendrogram of plant CuZnSOD amino acid sequences. The
predicted amino acid sequences were analyzed utilizing a DNASTAR
(Madison, WI) program, starting at Lys-Ala-Val-Ala-Val-Leu or a
homologous sequence to eliminate any amino-terminal transit sequences.
B, Alignment of the first 18 amino acids and conserved carboxy-terminal
amino acids of the proteins used for the dendogram in A to illustrate
cladistic relationships of the plant CuZnSODs. Underlined amino acids
indicate a putative peroxisomal targeting sequence. prx, Peroxisome;
cyt, cytosol; ec, extracellular. C, Dendogram of FeSOD and MnSOD amino
acid sequences. The amino acid sequences were analyzed from the start
of homology to the E. coli FeSOD sequence.