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Test of SOD antisera specificity. Twenty-five micrograms of total protein from Arabidopsis rosette leaves and 12 ng of the purified 6× His-tagged MSD1, FSD1, CSD1, CSD2, and CSD3 proteins were run on SDS-PAGE and subjected to immunoblot analysis. Postions of molecular-mass markers are listed on the left of each row of gels. The antisera and dilution (v/v) used are listed at the bottom of each gel. AP, Affinity-purified antiserum.
