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. 2012 Oct 23;8:78. doi: 10.1186/1744-8069-8-78

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Copyright ©2012 Chai et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Histological representation of injection sites within the RVM and Vi/Vc. Histological representation of the cannula tip placement within the RVM (A), the Vi/Vc transition zone (B), and Vc (C). Diagrams are separated according to the drug administered. RVM: L-733,060 and RP67580; Vi/Vc: IL-1β. IL-1ra, and Y-25130; Vc: ibotenic acid. Closed circles represent cannula tips accurately placed within the site of interest. Open circles represent cannula tips placed outside the site of interest. Dashed circles represent affected area from ibotenic acid injection, which involved dorsal mandibular and middle maxillary divisions. Animals with missed sites of injection showed no significant effect compared to control and were excluded from experimental groups. CN 7: facial nerve; sp5: Spinal trigeminal nucleus; Py; pyramidal tract; LRt: lateral reticulate nucleus.