Figure 5. Protein import into Chlamydomonas chloroplasts is not differentially regulated.

[³⁵S]Met-labeled precursors of Cr-prRBCS, Cr-prL11, prOE23, prTic40, and prL11 were imported into chloroplasts isolated from synchronized cultures of Chlamydomonas 1, 6, and 11 h after the start of the third light cycle and used for import experiments. Samples were analyzed by SDS-PAGE and autoradiography. An equal number of chloroplasts were loaded in each lane of the same gel. The amount of Chlamydomonas chloroplast Hsp70B was analyzed by immunoblotting and used for normalization of quantifications in the bar graph shown below each gel. The amount of mature protein imported at hour 1 was set as 100%. Data shown are mean ± SD, n = 3. m, mature form; p, precursor form; TR, in vitro–translated precursor proteins before import.