Figure 1. Construction of BCL2A1 lentiviral vectors, and demonstration of high level expression in producer and transduced cell line.(A) Schematic of the lentiviral vectors used for expression of murine BCL2A1a or human BCL2A1.

LTRs are self inactivating (SIN), ψ (packaging sequence), RRE (Rev-Responsive Element), PPT (Polypurine Tract), SFFV (Spleen Focus-Forming Virus promoter), IRES (Internal Ribosome Entry Site), HA (Hemagglutinine antigen), GFP (Green Fluorescent protein), PRE (post-transcriptional regulatory element). (B,C) Expression was assessed by western blot using an anti-HA antibody to recognize HA-tagged BCL2A1, or anti-GFP, and anti GAPDH as a loading control. Western blots were carried out in 293T producer cell lines (B) and in transduced BAF3 and 32Dcl3 cells (C).