Figure 2.

Time course of HKT1 expression in roots of cvs Jackson (lanes 1–5) and Klondike (lanes 6–12). Plants were transferred to complete nutrient inorganic nutrient medium containing 1.25 mm K⁺ on d 4. On d 7 plants were transferred to medium lacking K⁺ for 0 h (lanes 1 and 6), 4 h (lane 2), 6 h (lanes 3 and 7), 8 h (lane 8), 12 h (lanes 4 and 9), and 24 h (lanes 5 and 10), or to medium lacking N for 16 h (lanes 11 and 12). Fifteen micrograms of total RNA was loaded into each lane. Lanes were probed with a ³²P fragment of an HKT1 cDNA clone from wheat and reprobed with a ³²P fragment of rice 28S rDNA as an internal control for gel loading.