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. 2012 Nov 1;23(21):4226–4241. doi: 10.1091/mbc.E12-03-0210

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© 2012 Splinter et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Motility of Rab3C vesicles after BICD2-N recruitment. (A) Scheme of the regulated heterodimerization constructs used to attach BICD2-N to Rab3C-positive membranes. (B) Simultaneous live imaging of FKBP2-GFP-Rab3C (green) and mCherry–α-tubulin (red) in a transiently transfected MRC5-CV cell coexpressing HA–BICD2-N–FRB before and after rapalog addition; single frames are shown on the left, and projections of 40 frames are shown on the right. Imaging was performed with 100-ms interval/exposure using wide-field microscopy. Five consecutive frames were averaged. (C) Quantification of the number of FKBP2-GFP-Rab3C particle movements with length >1 μm. Ten cells were analyzed. (D) Distribution of FKBP2-GFP-Rab3C movement velocities to MT minus ends in MRC5-CV cells coexpressing HA–BICD2-N–FRB after rapalog addition. Approximately 90 events in 10 cells were analyzed.