Table 3. Effects of EDTA and sodium sulfite on R. padi transmission of purified cereal yellow dwarf virus-RPV using microinjection into the aphid hemolymph.
| Microinjection Experimenta | ||||||
| Infected Plant Homogenate Treatmentb | 1 | 2 | 3 | 4 | 5 | Total |
| Buffer only | 32/36 | 10/12 | 12/12 | 11/12 | 6/8 | 71/80 |
| Buffer + EDTA + Na2SO3 | 0/32 | 0/20 | – | – | – | 0/52 |
| Buffer + EDTA | – | – | 11/12 | 8/12 | 9/12 | 28/36 |
| Buffer + Na2SO3 | – | – | 2/12 | 0/12 | 1/12 | 3/36 |
a
Aphids were microinjected with purified luteovirus. For each experiment, the concentration of virus the same. Among different experiments, virus concentration varied from 20–60 µg/ml. Five experiments were performed using no additives, 2 experiments were performed using both sodium sulfite and EDTA, and 3 experiments were performed using only EDTA or sodium sulfite.
b
For each treatment, the numbers represent total number of plants infected/total number of plants inoculated. Three aphids previously microinjected with virus were placed on each healthy plant.