Figure 3. CD69 deficient DC do not have altered priming capacity.

A. CD69^+/+ or CD69^−/− Flt3l-derived BMDC were cultured with 0.5 µM CpG for 24 h. Overlays of CD69^+/+ (solid line) and CD69^−/− (dashed line) Flt3l-derived BMDC showing CD86 and CD40 expression on pDC (CD11c⁺, CD45RA⁺, left) and cDC (CD11c⁺, CD45RA⁻, right). B. and C. CD69^+/+ or CD69^−/− Flt3l-derived BMDC were pulsed with OVA at the indicated doses for 45 minutes, washed, and further cultured with OT-II (B.) or OT-I (C.) T cells for 3 or 2 days, respectively, in the presence of 0.5 µM CpG in duplicate. Percentage of proliferated Vα2⁺ CD4⁺ or Vα2⁺ CD8⁺ cells is depicted. Bars represent Standard Deviation (SD) of duplicate cultures. Experiments representative of two with similar results.