Figure 7. CD69 deficiency does not alter CD8⁺ T cell priming threshold at different peptide agonistic affinities.

Spleen CD8⁺ T cells were purified from CD69^+/+ (solid line) or CD69^−/− (dashed line) OT-I mice, stained with 2 µM CFSE and cultured at 0.5×10⁶ at 10∶1 with APC in the presence of the indicated doses of SIINFEKL (A), SIIGFEKL (B), and Catnβ1 (C) peptides. Graphs showing the number of proliferated (left column) and of CD25⁺ (right column) CD8⁺ T cells per well. The results are representative of two similar experiments with similar results.