Figure 4. 5-fluorouracil sensitivity assay.

A) Schematic representation of the chromosomal S. meliloti 1021 locus encompassing the annotation SMc01827. The arrows indicate possible transcription start sites (J.-P. Schlüter & A. Becker, personal communication). B) Bacterial growth was estimated by OD₆₀₀ measurements after 96 h in the presence of different concentrations of the uracil analogue. (•), wild type strain 2011; (○), Δhfq mutant strain 20PS01. Values represent the relative growth of S. meliloti 2011 and 20PS01 in presence and absence of the toxic analogue. Each bar shows average values for n = 3 replicate cultures ± SD. The experiment was repeated twice with similar results. The chemical structure of the uracil analogue is shown.