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| Target | Primers and probes | Sequences (5′-3′) |
| IFN-β (NM_010510) | Forward | CCCTATGGAGATGACGGAGAAG |
| Reverse | GAGCATCTCTTGGATGGCAAA | |
| Probe | TGCAGAAGAGTTACACTGC | |
| IL-12 p40 (NM_008352) | Forward | GCCAGTACACCTGCCACAAA |
| Reverse | TGTGGAGCAGCAGATGTGAGT | |
| Probe | AGGCGAGACTCTG | |
| IL-10 (NM_010548) | Forward | AGCATGGCCCAGAAATCAAG |
| Reverse | CGCATCCTGAGGGTCTTCAG | |
| Probe | AGCATTTGAATTCCCTGGGT | |
| β-actin (NM_007393) | Forward | CGATGCCCTGAGGCTCTTT |
| Reverse | TGGATGCCACAGGATTCCA | |
| Probe | CCAGCCTTCCTTCTT |
