Figure 3. In vivo dissemination of U112 and U112ΔiglB in Fischer 344 rats.
Rats were i.t. (panel A) or orally (panel B) vaccinated with either 105 CFU U112 or 107 CFU U112ΔiglB. At defined time points (days 3, 14, and 21 post vaccination), rats (n = 3 per group/time point) were euthanized for collection of lungs, spleens, and livers. Organs were homogenized and dilution plated on TSA plus cysteine to enumerate organ bacterial burdens. Significant differences in burden over the time course are noted (* denotes p<0.05, ** denotes p<0.01). Results are representative of two separate experiments.