Figure 5. Respiratory antibody response following mucosal vaccination.
Animals (n = 3 per group) were vaccinated i.t. (top panel) or orally (bottom panel) with either 105 CFU U112, 107 CFU U112ΔiglB, or mock vaccinated with PBS and rested for 28 days. Animals were euthanized to obtain bronchioalveolar lavage fluid (BALF) which was assayed by ELISA. Significant differences were observed between U112 and U112ΔiglB i.t. vaccinated and respective mock groups for total Ig and IgG2a (*p<0.05, **p<0.01). Animals primed with U112ΔiglB exhibited responses which were comparable to or higher than that observed for U112-primed rats. Horizontal lines represent mean of each group. Results are representative of two separate experiments.