Figure 4.

Amino acid sequence comparison of tomPRO1, tomPRO2, and other related genes. Predicted amino acid sequences of proB, proA, and P5CS genes were aligned using the multiple alignment program PILEUP, and the results were highlighted with the BOXSHADE program. Letters in the black and gray backgrounds indicate identical and similar residues, respectively. Representative regions that are highly conserved in P5CS proteins in plants but are either missing, divergent, or carry insertions in GK and GPR for tomPRO1 and in bacterial GK and GPR are underlined. Extended C-terminal tails of GK, which are conserved in most of prokaryotic GK, are shown by a dashed line. Abbreviations and accession numbers are provided in Methods.