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. 1998 Oct;118(2):661–674. doi: 10.1104/pp.118.2.661

Figure 6.

Figure 6

Complementation of a proBA mutation by tomPRO1 and tomPRO2, and their products expressed in E. coli. A and B, Expression vectors containing the tomPRO1 and tomPRO2 cDNA clones were introduced into strain KC1325 (a derivative of BL21[DL3]pLysS carrying the proB1658::Tn10 insertion, which is polar on proA). a, KC1325 harboring the vector, pKS only. b, KC1325 harboring pPRO1. c, KC1325 harboring pET32a only. d, KC1325 harboring pET32PRO2. Strain KC1325 containing each plasmid was streaked on minimal M63 medium containing Glc, thiamine, and IPTG with (A) and without (B) Pro, and incubated for 2 d at 37°C. All strains could grow on the media supplemented with Pro (A). C, Total cell extracts from either E. coli strain HB101, containing pKS (lane 1) and pPRO1 (lane 2), or strain KC1325, containing pET32a (lane 3) and pET32PRO2 (lane 4), were analyzed by SDS-PAGE. The gels were stained with Coomasie brilliant blue. tomPRO1 products are indicated as GK and GPR, and the tomPRO2 product as P5CS. Numbers at left refer to size standards (in kD).