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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Mol Cell Biochem. 2012 Aug 12;371(1-2):89–96. doi: 10.1007/s11010-012-1425-5

Figure 2. In situ Zymography in WT, CBS, GABA-A and double KO mice.

Figure 2

Figure 2

A: DQ- gelatin (a MMP substrate) was injected in tail vein 24- hours prior to in vivo imaging of the brain. In addition, FITC-labeled gelatin was infused through carotid artery prior to sacrifice the animal. The in fluorescence image of isolated brain was obtained in (A) WT; (B) CBS−/+; (C) GABA-A−/−; (D) CBS−/+ / GABA-A−/−. The arrows indicate MMP activity. B: in situ MMP activity in the brain. Cross-sections were prepared and MMP activiy was measured in tissue sections under confocal microscopein WT; CBS−/+; GABA-A−/−; CBS−/+ / GABA-A−/−. The arrows indicate capillaries and MMP activity. C: Densitometric analysis of MMP activity. *P<0.05 vs WT, **P<0.05 vs CBS−/+ ; n=4 animals/group.