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At 4 months, RT-PCR demonstrates persistently upregulated CNTF expression (right) from the denervated TA in the animals who had received CNTF-MSC injections. In comparison, the animals treated with MSCs alone (middle) and those animals injected with saline (left) demonstrated no upregulation of CNTF. The arbitrary values of mRNA expression were determined based on GAPDH as a standard internal control.
