Effects of oncogene silencing on the cell apoptosis, angiogenesis and cell proliferation in vivo. (a) Relative HDM2, c-my and VEGF mRNA level in the H460 tumor tissues. Columns, mean (n = 5); bars, SD; (b) CD31 staining of microvessels in H460 tumor treated with different formulations (original magnification × 200). (c) PCNA-positive nuclei in H460 tumor treated with different formulations (original magnification × 200). (d) TUNEL assay of H460 tumor treated with different formulations (original magnification × 100). Quantitation of the image data is shown in the histograms on the right. *, p < 0.05; **, p < 0.01; ***, p < 0.001.