Fig. 6.

Cellular morphology, as measured by cell cross-sectional area [(a) and (b)] and nuclear-to-cytoplasmic ratio [(c) and (d)] remains unchanged ($p>0.05$) from in vivo (IV) values within both the spinosum and basal epithelial layers of tissues maintained in live culture over 48 h and in frozen-thawed (F) tissues ($\text{mean}\pm \mathrm{SE}$ of $n=10$ [$n=6$ for (b) and (d)] measurements of a minimum of 20 cells per image).