Northern analysis of changes in the level of
expression of AOX2 (A) and AOX3
transcripts (B) during the development of soybean cotyledons. Each lane
contained 2 μg of poly(A+)-enriched RNA. Numbers across
the top refer to seedling age in days postimbibition. The position of
18S rRNA on each blot is indicated. Results from one of two independent
experiments are shown. C, Relative abundance of AOX
mRNAs as determined using a competitive reverse transcriptase-PCR
assay. The results from one experiment are shown. For d 5, 10, and 20,
n = 3, and for d 7 and 14, n =
1. Bars indicate se.