Figure 1.

Isolation of plasminogen receptors. Monocyte (Hoxa9-ER4) progenitor cells were differentiated with macrophage colony stimulating factor (M-CSF), which induces plasminogen receptors (▲) on these cells. Then intact cells were biotinylated (●) and passed over a dead cell removal column. Live cells were then lysed and membrane fractions prepared and passed over a plasminogen-Sepharose affinity column and specifically eluted. Biotinylated plasminogen receptors (▲●) were then bound to an avidin column and digested with trypsin. This figure was originally published in [17].