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. 2012 Oct 24;122(11):4218–4230. doi: 10.1172/JCI59176

Figure 3. The role of Cx40 in HPV is independent of Cx43.

Figure 3

(A) Expression of vWF (green) and Cx43 (red) and merged images for pulmonary arterioles (arrows) and capillaries (arrowhead) of Cx40+/+ and Cx40–/– mice. Scale bar: 50 μm. (B) Representative Western blots and quantification showing expression and Ser368 phosphorylation of Cx43 in fresh lung homogenate from Cx40+/+ and Cx40–/– mice. GAPDH and β-actin are shown as loading controls. (C) Group data (n = 5 lungs each) showing acute HPV response, determined as ΔPAP 10 minutes after onset of hypoxia (1% O2) versus normoxia (21% O2) in isolated perfused lungs of untreated Cx40+/+ mice and of Cx40+/+ mice treated with gap2740 (200 μM), gap2743 (200 μM), or both in combination. *P < 0.05 vs. control; #P < 0.05 vs. gap2740; †P < 0.05 vs. gap2743.