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RNA expression of (A) PN1, (B) GLI1, (C) CYCLIN D, and (D) PTCH1 after transfection of a PN1 expression plasmid or empty vector (2 μM) and treatment with cyclopamine (10 μM) in PC3 cells. One-way ANOVA (n = 4). *P ≤ 0.05.
