Figure 3. PN1-dependent reduction of Hh signaling requires RCL activity and the LRP receptor.

(A) Treatments as in Figure 2. Hh ligand, SHH, IHH, and DHH expression. Corresponding densitometry (B) confirms changes in SHH protein levels, and RT-PCR (C) shows SHH transcripts. One-way ANOVA (n = 3). RT-PCR and immunoblotting show effect on (D) SHH and (E) Gli of transfection with PN1 bearing the indicated mutations in LRP or RCL. One-way ANOVA (n = 3). *P ≤ 0.05.