Skip to main content
. 2012 Oct 8;122(11):4025–4036. doi: 10.1172/JCI59348

Figure 4. MMP9 levels control PN1 expression and indirectly regulate SHH levels.

Figure 4

(A) In PC3 cells, overexpression of PN1 reduces SHH levels. Furthermore, in a PC3-derived line lacking MMP9, PN1 was increased while SHH levels were lowered. (B) WT or mmp9–/– mouse tissues were immunoblotted for changes in SHH expression. Immunofluorescence of prostate tissue (C) and pancreas tissue (D) from WT and mmp9–/– mice of downstream Hh pathway proteins Gli1 and Ptch1. PN1 staining (green) and GLI1/PTCH1 (red) as indicated. Original magnification, ×20.