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. 2012 Oct 24;122(11):4172–4189. doi: 10.1172/JCI63377

Figure 2. Targeted disruption of glucocorticoid signaling in osteoblasts affects acute glucocorticoid signaling in osteoblasts but not muscle or liver as shown by qRT-PCR.

Figure 2

(A and B) Quantitative RT-PCR analysis of tibia RNA expression in WT and Tg mice treated for 3 days with either placebo or corticosterone of genes known to be modulated by glucocorticoids: (A) Gilz and (B) Fkbp5. (C and D) Quantitative RT-PCR analysis of muscle RNA expression of (C) Gilz and (D) Fkbp5 in WT and Tg mice treated for 3 days with placebo or corticosterone. (E and F) Quantitative RT-PCR analysis of liver RNA expression of (E) Gilz and (F) Fkbp5 in WT and Tg mice treated for 3 days with placebo or corticosterone. *P < 0.05, #P < 0.01, †P < 0.001 compared with respective genotype placebo-treated controls; **P < 0.01 WT 1.5 mg GC compared with Tg 1.5 mg GC (2-way ANOVA followed by post-hoc analysis; error bars represent SEM).