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. 2012 Oct 1;122(11):4118–4129. doi: 10.1172/JCI63606

Figure 6. TLR7 signaling in inflammatory cells regulates pancreatic cancer progression.

Figure 6

2-month-old p48Cre;KrasG12D mice were irradiated and made chimeric by bone marrow transfer from WT or Tlr7–/– mice. (A) 7 weeks later, mice were treated with either saline or caerulein to accelerate carcinogenesis. Mice were then sacrificed 3 weeks later, and pancreata were assessed by H&E and Ki67 staining. Original magnification, ×10 (H&E); ×60 (Ki67). Scale bars: 300 μm (H&E); 50 μm (Ki67). (B) The fraction of metaplastic and dysplastic ducts was measured for each cohort, and (C) the number of foci of invasive cancer was quantified using CK19 immunohistochemistry (n = 5 per group). *P < 0.05; ***P < 0.001. (D) Additional cohorts of chimeric mice (n = 4 per group) were not treated with caerulein, but kept until 12 months of life for histological analysis. Representative H&E-stained paraffin-embedded sections are shown. Original magnification, ×10. Scale bar: 300 μm.